Influence of the hollow-fibre membrane on the stability of -galactosidase and on lactose hydrolysis Kinetic models including adsorption of the enzyme onto the membrane
نویسنده
چکیده
An adsorption-kinetic model has been proposed to explain -galactosidase stability in a recirculation hollow-fibre bioreactor at different temperature. The kinetic constants of the model and its dependence on temperature have been evaluated. In addition, a study has been made of the influence of lactose and enzyme concentration on lactose hydrolysis with hollow-fibre modules m s a m a ©
منابع مشابه
Evaluating the Sintering Temperature Control Towards the Adsorptivity of Ammonia onto the Natural Zeolite Based Hollow Fibre Ceramic Membrane
The disproportionate quantity of ammonia presence in water has led to serious drinkable water scarcity worldwide. The abundant source of mineral and superior cations selectivity has made natural zeolite as a good adsorbent for the ammonia removal. This work aims to fabricate natural zeolite based hollow fibre ceramic membrane (HFCM) via extrusion based phase inversion and sintering techniques f...
متن کاملKinetic model for lactose hidrolysis in a recirculation hollow-%bre bioreactor
Enzymatic hydrolysis of lactose by -galactosidase (Lactozym) has been studied in a jacketed stirred-tank reactor (JSTR) and in a recirculation hollow-%bre bioreactor (RHFB). The hollow-%bre module was manufactured with modi%ed cellulose-acetate membrane (Cuprophan). The variables tested were: temperature (25–40◦C), lactose concentration (0.0278–0:208 M) and enzyme concentration (0.100–4:51 g l−...
متن کاملBioaffinity Based Immobilization of Almond (Amygdalus communis) b-galactosidase on Con A-layered Calcium Alginate-cellulose Beads: Its Application in Lactose Hydrolysis in Batch and Continuous Mode
In this study, immobilization of partially purified almond (Amygdalus communis) β-galactosidase on Con A layered calcium alginate-cellulose beads was investigated. Immobilized β-galactosidase retained 72% of theinitial activity after crosslinking by glutaraldehyde. Both soluble and immobilized enzyme exhibited the samepH and temperature optima at pH 5.5 and 50ºC, respectively. Howev...
متن کاملImmobilization of Urease onto Modified Egg Shell Membrane through Cross Linking
Background: Immobilization is an approach in industry to improve stability and reusability of urease. The efficiency of this technique depends on the type of membrane and the method of stabilization. Methods: The PEI-modified egg shell membrane was used to immobilize urease by absorption and glutaraldehyde cross-linking methods. The membranes were characterized by Fourier-transform infrared sp...
متن کاملKinetic modeling of methylene blue adsorption onto acid-activated spent tea: A comparison between linear and non-linear regression analysis
The kinetic study of methylene blue (MB) adsorption using acid-activated spent tea (AAST) as an adsorbent from aqueous solution with the aim of comparing linear and non-linear regression analysis methods was performed at varying initial MB concentrations (10-100 mg/l). Hence, spent tea leaves, which were activated using concentrated sulfuric acid, were prepared. The physicochemical characterist...
متن کامل